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고온조건에서 등숙이 양호하고 도열병에 강한 조생 고품질 벼 ‘중모1024’ 육성

Blast Resistant Early Maturing Rice ‘Jungmo1024’ with High Temperature Tolerance during Grain Filling Stage

Ji-Ung Jeung1,*, Young-Seop Shin1, Im-Soo Choi1, Jae-Ki Chang1, Myeong-Ki Kim1, Jeom-Ho Lee1, Hyang-Mi Park1, Chang-Ihn Yang1, Yong-Hee Jeon1, Jung-Pil Suh2, In-Bae Choi1, Jong-Min Jeong1, Nak-Sig Sung1, Jeong-Heui Lee1, Mi-Ra Yoon1, Chung-Kon Kim3
The Korean Journal of Breeding Science 2016;48(1):72-84.
Published online: February 29, 2016

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*Corresponding author (jrnj@korea.kr, +82-63-238- 5236, +82-63-238-5205)
• Received: November 20, 2015   • Revised: December 1, 2015   • Accepted: December 2, 2015

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Blast Resistant Early Maturing Rice ‘Jungmo1024’ with High Temperature Tolerance during Grain Filling Stage
Korean. J. Breed. Sci.. 2016;48(1):72-84.   Published online March 31, 2016
DOI: https://doi.org/10.9787/KJBS.2016.48.1.072
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Blast Resistant Early Maturing Rice ‘Jungmo1024’ with High Temperature Tolerance during Grain Filling Stage
Korean. J. Breed. Sci.. 2016;48(1):72-84.   Published online March 31, 2016
DOI: https://doi.org/10.9787/KJBS.2016.48.1.072
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Blast Resistant Early Maturing Rice ‘Jungmo1024’ with High Temperature Tolerance during Grain Filling Stage
Image Image Image Image
Fig. 1. Pedigree diagram of ‘Jungmo1024’.
Fig. 2. Genealogical diagram of ‘Jungmo1024’.
Fig. 3. Phenotypic comparison between ‘Namil(Suweon472)’, the wild type, and it’s induced mutant, ‘Jungmo1024(Suweon535)’.
Fig. 4. Reactions of ‘Jungmo1024’ against rice blast, Magnaporthe grisea. A) Strong resistance of ‘Jungmo1024’ observed during the field blast nursery test conducted at Cheolwon substation, NICS, RDA in 2009. It should be noticed that ‘Odea’ has been placed as the major rice cultivar cultivated in mid-north middle plain region of South Korea including Cheolwon area since 1990s. B) High durability of ‘Jungmo1024’ at the end of 7th sequential planting with co-infection of 30 virulent M. grisea isolates along with ‘Nagdong’, highly susceptible cultivar and ‘Namil’, the wild type of ‘Jungmo1024’ (see also Table 4).

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Blast Resistant Early Maturing Rice ‘Jungmo1024’ with High Temperature Tolerance during Grain Filling Stage

Comparison between ‘Jungmo1024’ and the wild type, ‘Namil’ on the major agronomic traits.

Variety Heading date (mm.dd) Culm length (cm) Panicle length (cm) No. of panicles /hill No. of spikelets /panicle Ratio of ripened grain(%) 1,000-grain weight of brown rice(g) Milled Rice yield (kg/10a)
Jungmo1024 (Suweon535) 7.31** 66** 19ns 17** 77** 86.1** 21.3 ** 548**
Namil 8.02 75 22 11 119 76.5 25.7 426

zThe data set are derived from the replicated yield trial conducted in 2009 at NICS, RDA, Suwon.

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Comparisons of early-maturing Korean japonica rice cultivars in terms high-temperature tolerance during grain filling stage.z

Cultivars Green House (High-Temp.)y Paddy field (Ordinary temperature)
Head ricex (%) Heading Date (month.day) Mean temp. of ripeningw (°C) Head ricex (%) Heading Date (month.day) Mean temp. of ripeningw (°C)
Jungmo1024 (Suweon535) 39.7a 8.04 28.4 82.4a 8.02 25.9
Goun 30.3b 7.29 28.5 75.2a 7.28 26.2
Jinbu 23.0c 7.30 28.4 76.3a 7.29 26.2
Odae 24.1c 7.29 28.4 64.7b 7.29 26.1
Ungwang 19.0c 8.03 28.4 66.8b 8.02 26.0

zThe experiment was conducted during two years (2009~2010) at National Institute of Crop Science, Suwon (37°16′N, 126°59′E, 37 m altitude), Korea.

yGreen house was closed after transplanting the seedlings to have high-temperature condition.

xFive to seven grams of brown rice per each rice cultivar was used to estimate the head rice percentage by using a near-infrared spectrometric analyzer (FOSS Cervitec 1625 Grain Inspector). Means with the same letter are not significantly different by Duncan’s multiple range test at 5% level.

wAfter checking the heading date for each cultivar, the mean temperature of ripening stage was calculated by averaging the daily mean temperature for 40 days.

Major agronomic traits and yield components of ‘Jungmo1024’ along with the check variety, ‘Hwaseong’.z

Variety Heading date (mm.dd) Culm length (cm) Panicle length (cm) No. of panicles /hill No. of spikelets /panicle Ratio of ripened grain(%) 1,000-grain weight of brown rice(g)
Jungmo1024 7.29** 69** 20ns 16* 82** 78.5* 22.3 ns
Hwaseong 8.10 84 21 14 93 81.8 22.4

zThe over-all means of three years local adaptability trials (2010~2012) conducted in ‘Suwon’.

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Resistance reactions to blast disease.

Variety Reaction to leaf blast at blast nursery testsz Area ratio of leaf lesion of Magnaporthe grisea during sequential plantings (%)y Reaction to neck blast(%)x
No. of tested sites (14) 1 2 3 4 5 6 7 Resistance (0~7) No. of susceptible isolate (30) Jecheon Iksan
R (0~3) M (4~6) S (7~9) Mean
Jungmo1024 12 2 0 2.4 0.5 0.5 0.5 0.3 0.1 0.1 0.0 0 1 0.2 0.0
Hwaseong 0 12 2 5.8 58 58 48 50 50 38 32 7 18 8.7 11.0

zN fertilizer of blast nursery test = 240 kg/ha

yDurability against co-infection of 30 virulent M. grisea isolates during sequential planting (Kim et al. 2004)

xN fertilizer of neck blast = 220 kg/ha

R: resistance, M: moderately resistance, S: susceptible.

Reaction to bacterial blight, virus disease and insect pests.

Variety Bacterial blight Viral diseases Resistance to insectsz
K1 K2 K3 K3a Stripe (%) Dwarf Black-streak dwarf BPH WBPH SBPH
Jungmo1024 S S S S S(100) S S S S S
Hwaseong S S S S R(14.1) S S S S S

zBPH(brown planthopper), WBPH(white-backed planthopper), SBPH(small brown planthopper).

R: resistance, S: susceptible.

Response to physiological and abiotic stresses.

Variety Premature heading (%) Occurrence of wilting Leaf senescence at maturing Cold tolerancez Viviparous germinationx (%)
Leaf discolorationy (1~9) Heading delay (day) Grain Fertility (%) Phenotypic Acceptability (1~)
Jungmo1024 13.9** Tolerance Normal 1 11ns 39ns 6 45.4ns
Hwaseong 0.0 Tolerance Slow 1 11 53 6 17.1

zCold tolerance was evaluated by using cold-water (17°C) irrigation nursery in Chuncheon subsbtaion, NICS.

yEvaluation at maximum tillering stage (1: toterance; 9: susceptible).

xGermination rate under water saturated incubation (17°C) for 7 days on the panicle at 40 days after pollination.

Not significant(ns) in the t-test, while * and ** significant at *P < 0.05 and P < 0.01, respectively.

Culm characters related to lodging.z

Variety Culm length (cm) Length of 3rd internode (cm) Breaking Resistancey (g) Fresh weight of upper portion (g) Bending momentx (g.cm) Lodging Indexw
Jungmo1024 74** 13* 610* 11.3* 1,075** 176**
Hwaseong 85 11 561 14.7 1,597 298

zEvaluated under ordinary transplanting nursery condition, and the evaluation methods was based on Kim et al.(2000).

yBreaking resistance of the middle point of 3rd internode with leaf sheath.

xLength from the lower node of 3rd internode to the top of panicle (fresh weight of upper portion + fresh weight of 3rd internode)

wBending moment / Breaking resistance × 100

*and ** Significant at *P < 0.05 and P < 0.01, respectively in the t-test.

Characteristics related to grain shape and quality.

Variety Brown rice Translucency (1~9) White belly/core (0~9) Alkali digestive value (1~7) Protein content (%) Amylose content (%) Palatability of cooked rice (-3~+3)
Length (mm) Width (mm) L/W ratio
Jungmo1024 5.21** 2.73ns 1.91ns 1 0/0 6.2 6.2 18.4* 0.06
Hwaseong 4.98 2.79 1.79 1 2/0 6.5 6.9 20.6 0.08

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Characteristics related to milling quality.

Va｣riety Milling recovery ratio (%) Head rice milling recovery ratio (%)
Brown/rough Milled/brown Milled/rough Head Rice
Jungmo1024 82.1ns 89.5ns 73.4* 93.7** 68.8 **
Hwaseong 83.2 89.9 74.8 73.8 55.2

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Summary on the yield potential evaluated during 3 years local adaptability trials (2010~2012).

Culture Seasonz (no.) Regiony Trial sites Milled Rice Yield (MT/ha) Index (A/B) × 100
Jungmo1024 (A) Check varietyx (B)
2010 2011 2012 Mean 2010 2011 2012 Mean 2010 2011 2012 Mean
Ordinary Planting Culture (11) MDP Suwon 4.66 4.41 4.81 4.63 4.77 4.59 4.90 4.75 hs 98 96 98 97
Hwaseong 4.22 5.05 5.75 5.01 4.37 4.39 5.32 4.69 hs 97 115 108 107
Yeoju 4.63 5.29 5.52 5.15 5.41 5.02 5.10 5.18 hs 86 105 105 99
Yeoncheon 4.38 4.45 nt 4.42 4.77 4.86 4.79 4.81 hs 92 92 - 92
Chuncheon 5.89 5.29 5.60 5.59 5.06 5.11 5.97 5.38 hs 116 104 94 104
Mean 4.76 4.90 5.42 4.96 4.88 4.79 5.22 4.96 hs 98 104 102 100
MSM Boeun 4.66 4.42 nt 4.54 4.58 5.09 5.31 4.99 hs 102 87 - 91
Jinan 4.88 4.56 4.57 4.67 5.15 5.15 4.93 5.08 hs 95 89 93 92
Andong 5.62 6.54 5.46 5.87 5.89 5.90 5.92 5.90 hs 95 111 92 99
Mean 5.05 5.17 5.02 5.03 5.21 5.38 5.39 5.32 hs 97 96 93 94
MWC Namyang 4.55 3.40 5.25 4.40 5.18 3.93 5.71 4.94 hs 88 87 92 89
MMP Cheolwon 5.19 5.55 4.87 5.20 4.69 5.03 5.23 4.98 od 111 110 93 104
NEC Ganreung 5.51 5.35 5.15 5.34 4.67 5.31 4.80 4.93 od 118 101 107 108
Grand Mean 5.04 4.94 5.33 5.10 hs 98 100 98 98
4.93 4.94 5.22 4.98 4.68 5.17 5.02 4.96 od 105 96 104 101
Early Planting Culture (3) MDP Suwon nt 5.77 4.80 5.29 nt 5.01 5.12 5.07 jp - 115 94 104
HNP Iksan nt 4.62 4.76 4.69 nt 4.91 4.95 4.93 jp - 94 96 95
YNP Milyang nt 4.87 5.11 4.99 nt 4.91 5.27 5.09 jp - 99 97 98
Grand Mean - 5.09 4.89 4.99 - 4.94 5.11 5.03 jp - 103 96 99
Late Planting Culture (3) MDP Suwon nt nt 4.68 4.68 nt nt 4.95 4.95 go - - 95 95
HNP Iksan nt nt 3.89 3.89 nt nt 3.31 3.31 go - - 118 118
YNP Milyang nt nt 4.70 4.70 nt nt 4.41 4.41 go - - 107 107
Grand Mean - - 4.42 4.42 - - 4.22 4.22 go - - 105 105

zLocal adaptability trials for early and late planting cultures were conducted 2(2011, 2012) years and 1(2012) year, respectively. The total numbers of trial sites for each culture season are indicated in parenthesis.

yRegions are middle plane (MDP), mid-south mountain (MSM), mid-west coastal (MWC), mid-north middle plain (MMP), north-east coastal (NEC), honam plane (HNP), and Yeongnam plane (YNP).

xCheck varieties for each trial site are indicated as superscripted abbreviation with the mean milled rice yield; ‘Hwaseong’ (hs), ‘Odae’ (od), ‘Jopyeong’ (jp), and ‘Geumo’ (go).

Table 1. Comparison between ‘Jungmo1024’ and the wild type, ‘Namil’ on the major agronomic traits.

The data set are derived from the replicated yield trial conducted in 2009 at NICS, RDA, Suwon.

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Table 2. Comparisons of early-maturing Korean japonica rice cultivars in terms high-temperature tolerance during grain filling stage.z

The experiment was conducted during two years (2009~2010) at National Institute of Crop Science, Suwon (37°16′N, 126°59′E, 37 m altitude), Korea.

Green house was closed after transplanting the seedlings to have high-temperature condition.

Five to seven grams of brown rice per each rice cultivar was used to estimate the head rice percentage by using a near-infrared spectrometric analyzer (FOSS Cervitec 1625 Grain Inspector). Means with the same letter are not significantly different by Duncan’s multiple range test at 5% level.

After checking the heading date for each cultivar, the mean temperature of ripening stage was calculated by averaging the daily mean temperature for 40 days.

Table 3. Major agronomic traits and yield components of ‘Jungmo1024’ along with the check variety, ‘Hwaseong’.z

The over-all means of three years local adaptability trials (2010~2012) conducted in ‘Suwon’.

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Table 4. Resistance reactions to blast disease.

N fertilizer of blast nursery test = 240 kg/ha

Durability against co-infection of 30 virulent M. grisea isolates during sequential planting (Kim et al. 2004)

N fertilizer of neck blast = 220 kg/ha

R: resistance, M: moderately resistance, S: susceptible.

Table 5. Reaction to bacterial blight, virus disease and insect pests.

BPH(brown planthopper), WBPH(white-backed planthopper), SBPH(small brown planthopper).

R: resistance, S: susceptible.

Table 6. Response to physiological and abiotic stresses.

Cold tolerance was evaluated by using cold-water (17°C) irrigation nursery in Chuncheon subsbtaion, NICS.

Evaluation at maximum tillering stage (1: toterance; 9: susceptible).

Germination rate under water saturated incubation (17°C) for 7 days on the panicle at 40 days after pollination.

Not significant(ns) in the t-test, while * and ** significant at *P < 0.05 and P < 0.01, respectively.

Table 7. Culm characters related to lodging.z

Evaluated under ordinary transplanting nursery condition, and the evaluation methods was based on Kim et al.(2000).

Breaking resistance of the middle point of 3rd internode with leaf sheath.

Length from the lower node of 3rd internode to the top of panicle (fresh weight of upper portion + fresh weight of 3rd internode)

Bending moment / Breaking resistance × 100

and ** Significant at *P < 0.05 and P < 0.01, respectively in the t-test.

Table 8. Characteristics related to grain shape and quality.

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Table 9. Characteristics related to milling quality.

Not significant (ns) in the t-test, while * and ** Significant at *P < 0.05 and P < 0.01, respectively.

Table 10. Summary on the yield potential evaluated during 3 years local adaptability trials (2010~2012).

Local adaptability trials for early and late planting cultures were conducted 2(2011, 2012) years and 1(2012) year, respectively. The total numbers of trial sites for each culture season are indicated in parenthesis.

Regions are middle plane (MDP), mid-south mountain (MSM), mid-west coastal (MWC), mid-north middle plain (MMP), north-east coastal (NEC), honam plane (HNP), and Yeongnam plane (YNP).

Check varieties for each trial site are indicated as superscripted abbreviation with the mean milled rice yield; ‘Hwaseong’ (hs), ‘Odae’ (od), ‘Jopyeong’ (jp), and ‘Geumo’ (go).

Table 1.

Table 2.

Table 3.

Table 4.

Table 5.

Table 6.

Table 7.

Table 8.

Table 9.

Table 10.

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