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Soybean Variety, ‘Hanol’ Adaptable for Double Cropping System with Early Maturing and Large Seed

Jong Min Ko1, In Youl Baek1, Won Young Han1, Yeong Hoon Lee1, Byoung Won Lee1, Beom Kyu Kang1,*, Hyun Tae Kim1, Jung Kyung Moon1, Tae Joung Ha1, Sang Ouk Shin1, Ki Won Oh1, Seuk Ki Lee1, Min Jung Seo1, Dal Soon Kang2, Kyu Hwan Choi3, Yong Duk Kim4

Published online: November 30, 2016

1 농촌진흥청 국립식량과학원,

1 National Institute of Crop Science (NICS), RDA, Jeonju 55365, Korea

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*Corresponding author (hellobk01@korea.kr, +82-53-663-1120, +82-53-663-1101)
• Received: May 18, 2016   • Accepted: October 6, 2016

© The Korean Society of Breeding Science

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  • Early maturity of soybean (Glycine max (L.) Merr.) is an important character for double cultivating system with winter crops such as onion or garlic in southern area of Korea. Soybean cultivars released in the past, especially ‘Keunolkong’ was widely cultivated but it was weak at abiotic stress and low yield potential because of too short growing period and short stem height. ‘Hanol’, a new early maturing cultivar showing better abiotic stress tolerant and higher yield potential, was developed from the cross between SS91408 and ‘Hwaeomputkong’, and released in 2009. ‘Hanol’ is, compared to ‘Keunolkong’, Hanol’s growing period is five days longer and its height is 5cm higher. In addition, it showed better excess-water tolerant and 26.5g 100-seed weight. At regional adaptation yield trials (RYT) in six regions from 2007 to 2009, ‘Hanol’ showed 2.04 tons of seed per hectare, 14% higher than ‘Keunolkong’ (1.80ton/ha). ‘Hanol’ is promising for double cropping system with higher yield.
콩[Glycine max (L.) Merr.]은 된장, 간장, 두부, 콩나물 등 한국 식단에 매우 밀접한 주재료이다. 또한 단백질 함량이 높고 필수아미노산의 구성이 우수하여 육류의 대체식품으로 관심을 받고 있다. 하지만 재배면적은 56,666ha(KOSIS 2015), 사료용 을 제외한 자급률은 35.9%(MAFRA 2015)에 머무르고 있어 국내 콩 생산량을 높여 먹거리의 질 향상과 안정적인 국산콩 공급을 도모할 필요가 있다.
다양한 작물과의 작부체계를 통해 경지이용도를 높여 수익을 향상하는 것은 제한된 면적에서 국산콩 생산을 장려하기 위한 방법 중 하나이다. 콩은 밀, 보리, 양파, 마늘과 같은 동계작물 또는 감자, 고구마, 담배, 옥수수 등 봄·여름작물과 재배할 수 있으며 특히 남부지방의 ‘맥류+콩’ 또는 ‘마늘·양파+콩’ 작부체계는 우리나라 밭의 경지이용도 제고와 농가소득향상에 크게 기여하고 있다(RDA 2015).
맥류, 마늘, 양파와 같은 동계작물과 작부체계를 구성할 때는 각 작목의 파종기 및 수확기가 겹치지 않고 다음 작목을 준비할 수 있는 시간을 확보하는 것이 중요하다. 이에 적응하기 위해서 생육기간이 100일 내외로 짧은 것이 유리하다. 이와 같은 단기성 콩 품종에는 1992년에 재래종에서 선발된 ‘큰올콩(Lee et al. 2015)’이 개발된 이후 ‘새올콩(Lee et al. 2015)’과 ‘다올콩(Lee et al. 2015)’, 검정콩에서는 ‘검정올콩(Lee et al. 2015)’과 ‘검정 새올콩(Lee et al. 2015)’ 등이 있으며 이들 품종은 생육기간이 100일 내외로 짧지만 수량성과 종실의 품질이 낮은 단점이 있어 이를 개량할 필요가 있다. 미국 성숙군 분류체계는 성숙기의 빠르고 느린 정도에 따라 적응할 수 있는 지역을 13개로 나누어 가장 빠른 품종이 적응할 수 있는 북쪽 지역인 MG 000(Maturity group 000)에서 가장 느린 품종이 적응할 수 있는 남쪽 지역 MG X까지 로마자 순서로 표시하고 있다(Boerma & Specht 2004, Edwards & Purcell 2005). 이 중 MG 00과 MG 0 수준의 숙기가 빠른 콩은 낮은 수량의 원인이 꼬투리에 종실이 차오르기 시작하는 시기인 R5 시기(Fher et al. 1971)의 온도, 강우, 한발 등 기상변동, 개화가 시작하는 R1 시기(Fher et al. 1971)와 R5 시기의 초관폐쇄형태와 관계가 있다(Kane & Grabau 1992, Kane et al. 1997a, Kane et al. 1997b). 초형을 개량하고 R5 이후 시기의 내재해성을 향상시킴과 더불어 적정 파종기 및 재식밀도 설정 등 재배기술을 함께 개발하면 생육기간이 짧으면 서도 수량을 안정적으로 높일 수 있을 것이다.
농촌진흥청 국립식량과학원에서는 동계작물과 이모작 작부 체계가 가능하고 수량성이 향상된 단기성 콩 품종 개발을 위해 노력한 결과, 평균 생육기간이 94일이며 대조품종인 ‘큰올콩’보 다 수량이 14% 증수된 ‘한올’을 개발하게 되어 그 육성경위와 주요특성을 소개하고자 한다.
‘한올’은 2007년도부터 2009년도까지 3년간 지역적응시험 (RYT, Regional Yield Trial)을 수행하였으며, 2007년과 2008 년에는 수원, 대구, 익산, 진주 등 4개소, 2009년에는 수원, 밀양, 익산, 진주, 제주 등 5개소로 총 6개 지역에서 농업과학기술 연구조사분석 기준에 따라 생육특성 및 수량성을 조사하고 ‘큰올 콩’과 비교하였다. 대구, 수원은 난괴법 4반복으로, 이외 지역은 난괴법 3반복으로 배치하였다. 지역별 파종기와 재식밀도는 단 작 지역(수원)에서는 5월 하순에 재식거리 60 x 15 cm로, 이모작 지역(대구, 익산, 밀양, 진주, 제주)에서는 6월 상중순에 60 x 10 cm로 파종하였고, 입모 후 1주 2개체로 솎아 주었다.
지역적응시험을 실시하기 전 수행한 생산력검정시험은 2005 년 실시한 생산력검정예비시험(PYT, Preliminary Yield Trial) 과 2006년 생산력검정본시험(AYT, Advanced Yield Trial)으 로 나누어 밀양에서 실시되었으며 생산력검정예비시험은 난괴 법 2반복배치, 생산력검정본시험은 난괴법 3반복 배치하여 농업 과학기술 연구조사분석 기준에 따라 생육특성과 수량성을 조사 하였다.
내병성 검정을 위해 5월 상순에 검정포장에 파종하여 콩모자 이크바이러스와 불마름병의 자연 이병율을 조사하고, 저항성 검정을 위해 각 시험 전년도에 접종 및 증식하여 저온 보관해둔 콩모자이크바이러스 G5(Lim et al. 2003), G6H(Seo et al. 2009), G7H(Kim et al. 2003) 균계를 지닌 잎을 마쇄하여 버퍼에 녹인 후 즙액을 만들어 초생엽 전개시 Carborandom을 이용하여 잎 표면에 상처를 낸 후 접종 하였다. 접종 후 2주 후 접종한 하위엽을, 10일 뒤 상위 엽을 병반 없음(-), 갈색 병반(L), 엽맥 병반(V), 모자익 증상(M), 고사(N), 5 가지 병징으로 판별 및 조사 하였다. 불마름병은 8ra를 증식하여 증류수에 희석하여 OD값 0.2-0.3으로 농도를 맞추어 제 2본엽기에 충분히 분무접종 하고 습식처리 후 10일이 경과하고 2회에 걸쳐 잎에 나타난 병징 발현을 조사하였다.
내도복성 검정을 위해 60 x 15 cm 의 표준 재식밀도와 60 x 7.5 cm의 2배 밀식재배를 동시에 수행하여 도복 정도를 조사하였으며, 협개열 정도는 성숙한 꼬투리를 40°C 건조기에 48시간 건조 후 꼬투리의 개열 정도를 조사하였다. 내습성 검정은 검정용 경사포를 이용하여 개화기경 10일간 담수처리 하여 피해가 없거 나 잎 큰부분이 약간 마르거나 황화될 경우 ‘1’, 잎이 1/4 정도 마르거나 황화되고 고사율 10% 이내일 때 ‘3’, 잎이 1/4~1/2 정도 마르거나 황화되고 고사율 20% 이내일때 ‘5’, 전체 잎의 1/2 이상 마르거나 황화되고 고사율 50% 이내일 때 ‘7’, 50% 이상의 개체가 고사되었을 때를 ‘9’로 하여 조사하였다.
종자의 단백질 함량은 질소 분석기(Elementar Analysen system, US/RapidN111, germany)를 이용하여 분석하였으며 지방함량 및 지방산 조성분석은 자동유지추출장치(Soxhlet System: BUCHI Labotechnik, B-811, AG, Switzerland)에 넣고 n-hexane으로 3시간 열수 추출한 후 지방함량을 구하였다. 그리고 지방산 조성 분석은 GC(gas chromatography)로 분석하 였다.
아이소플라본은 성분 분석을 위해 분쇄한 시료 1.0 g을 50% methanol 용액 20 mL에 12시간 실온에서 교반한 뒤 추출액을 여과지(Whatman NO. 2)를 이용하여 여과하였다. 여과된 시료 는 다시 HPLC 분석을 위해 0.45 μm 필터로 여과하였다. 이소플라 본 분석은 액체 크로마토그래피 (Agilent 1100, USA)로 분석하였다.
두부는 원료콩 150g을 10°C에서 15시간 침지 시킨 후 가수량 을 10배로 하여 3분간 와링브렌더로 마쇄하고, 마쇄한 두미를 여과포에 넣어 압착여과기로 압착(3psi)하여 두유를 얻고, 두유 를 스테인레스 용기(Φ15×30 cm)에 담아 가압솥에서 98~100°C 로 10분간 가열한 후, 두유를 85°C로 유지시키면서 CaSO4를 2% 첨가하고, 20분간 그대로 정치하여 응고시켰다. 응고물은 두부성형틀에 넣고(12×10×4 cm) 압착성형 하였고, 압착된 두부 를 15°C 정도의 물에 2시간 정도 수침시킨 후 완성된 두부를 시료로 사용하여 물성·색차 및 수율을 조사하였다.
청국장은 원료콩 20g을 15시간 침종 후 121°C에서 15분간 살균하고 청국장 균(bacillus subtilis) 200μl를 접종하여 유산균 발효기(㈜엔유씨전자, NY-3200S)에 치상하였다. 24시간 뒤 끈 적임 정도를 대원콩을 ‘3’으로 하여 1(약)-5(강) 수준으로 평가하 고, 점질물(γ-polyglutamate)량은 청국장 5g에 증류수 30ml를 가하여 낟알이 부서지지 않게 저어 추출한 후 15,000×g에서 10분간 원심분리하여 얻은 상층액을 동결 건조하여 중량을 측정 하였다.
메주는 원료콩 2kg을 세척 후 9시간 동안 수침한 후 5분동안 절수하고 115°C에서 40분간 증자하였다. 증자 후 콩을 압력솥에 서 꺼낸 후 넓게 펴 식힌 다음 회전식 스크류 쵸파로 마쇄하고 목제 성형틀에 넣어 성형한 후 30±2°C, 70±5% RH의 항온항습 실에서 40일 동안 발효시켰다. 발효된 메주는 실외 건조 후 무게를 측정하였다.
두부가공 및 메주·청국장 발효 적성은 ‘큰올콩’과 비교 분석 하였다(RDA 2012). 기타 시비관리와 생육특성 조사는 농진청 신품종개발공동연구 계획서 (RDA 2009a)의 기준에 의하여 실 시하였다.
육성 과정
‘한올’은 조숙 다수성 콩 신품종육성을 목표로 내재해 조숙 대립인 ‘SS91408’을 모본으로, ‘화엄풋콩’을 부본으로 1999년 에 교배한 것으로, 2000년부터 F1, F2 세대를 양성하고, F3부터 계통육종법으로 선발한 SS99408-2B-20-3-2계통이다. 2005년 부터 2년간 실시한 생산력검정시험에서는 조숙 다수성으로 유망 시되어 ‘밀양184호’의 계통명을 부여한 후 2007년부터 2009년 까지 3년간 지역적응시험을 실시한 결과, 조숙 내병 다수성인 특성을 지니면서 이모작이 가능한 품종으로 인정되어 2009년 12월 농작물 직무육성 신품종 선정위원회에서 전국에서 재배 가능한 신규등록품종으로 결정하고 ‘한올’로 명명하였으며 (RDA 2008, 2009b, 2010) 육성과정은 Fig. 1과 같다.
Fig. 1.
Pedigree diagram of ‘Hanol’. a; PYT: Preliminary yield trial, b; AYT: Advanced yield trial, c; RYT: Regional yield trial.
KJBS-48-4-492_F1.gif
식물학적 및 농업적 특성
‘한올’의 고유특성은 ‘큰올콩’과 매우 유사하다. 유한신육형 이며 엽형은 난형이고, 꽃색은 백색이다. 모용색은 회색이고, 협색은 담갈색, 종피색은 황색, 배꼽색은 옅은 갈색이며 종실모 양은 구형이다(Table 1).
Table 1.
Qualitative characteristics of ‘Hanol’
Table 1.
Cultivar Growth habit Leaflet shape Flower color Pubescence color Seed coat color Pod color Hilum color Seed shape
Hanol Determinate Oval White Gray Yellow Light brown Yellow Spherical
Keunolkong Determinate Oval White Gray Yellow Light brown Yellow Spherical
‘한올’의 개화기는 극조생종인 ‘큰올콩’보다 3일 늦은 7월 25일이며 성숙기는 5일 늦은 9월 14일이었다. 경장은 39cm로 대조품종보다 크며, 개체당 협수는 23개, 분지수는 2.1개였다. 성숙한 종실의 100립중은 26.4g으로 대립종이다(Table 2).
Table 2.
Quantitative characteristics of ‘Hanol’ determined by regional yield trial from 2007 to 2009.
Table 2.
Cultivar Flowering date Maturity date Stem height (cm) No. of nods No.of branch No. of pods per plant Weight per 100 seeds (g)
Hanol July 25 Sep. 14 39±5.2az 11±1.2a 2.1±0.89a 23±7.3a 26.4±2.31a
Keunolkong July 22 Sep. 9 34±4.7b 10±0.9a 2.0±0.71a 22±7.8a 27.9±2.46a

zValue of characteristics ± Standard Error Same letters in the column are not significantly different by DMRT at 5%.

‘한올’의 지역별 성숙기와 파종에서 수확까지의 전체 생육일 수를 보면 전국 지역적응시험 6개소 모든 지역에서 9월 20일까지 는 성숙기에 도달하였다. 한편 지역별 생육일수 범위는 86~99일 사이였으며 평균생육일수는 94일이었다. 육성 보급중인 콩 품종 중에서 숙기가 가장 빠른 ‘큰올콩’의 생육일수 89일에 비해서는 늦지만 9월 중순까지 수확이 가능하여 겨울철 동계작물인 양파, 마늘과 맥류의 2모작 작부체계가 가능하다(Table 3).
Table 3.
Maturing date and growing days of ‘Hanol’ on the regional yield trials carried out at six locations
Table 3.
Location Hanol (Month. Date)
 Keunolkong (Month. Date)
2007 2008 2009 Mean Growing Period (days) 2007 2008 2009 Mean Growing Period (days)
Suwon Sep.20 Sep.15 Sep.8 Sep.14 99 Sep.12 Sep.1 Sep.2 Sep.5 90
Daegu Sep.26 Sep.12 - Sep.19 99 Sep.14 Sep.12 - Sep.13 93
Miryang - - Sep.14 Sep.14 94 - - Sep.10 Sep.10 90
Iksan - Sep.22 Sep.18 Sep.20 86 - Sep.22 Sep.22 Sep.22 90
Jinju Sep.15 Sep.13 Sep.8 Sep.12 90 Sep.9 Sep.8 Sep.3 Sep.6 85
Jeju - - Sep.5 Sep.5 88 - - Sep.1 Sep.1 84
Mean Sep.20 Sep.15 Sep.14 Sep.14 94 Sep.11 Sep.10 Sep.7 Sep.9 89
내재해 및 내병충성
‘한올’은 내재해성 인위검정포장에서의 시험결과 도복은 발 생하지 않았고 습해와 한발해에 대한 내성은 ‘큰올콩’보다 우수 한 중간정도를 보였다. 내탈립성은 약하며, 콩나방 피해율은 0.7%였다(Table 4).
Table 4.
The response of ‘Hanol’ to abiotic stress and insect estimated from 2007 to 2009.
Table 4.
Cultivar Lodging (0-9)z Water stress (0-9)z
 Shattering y
 Damaged seed for Pod borer (%)
Excess water Drought Field(0-9) Ratios by Oven test(%)xz
Hanol 0 4 4 9 9 0.7
Keunolkong 0 8 5 9 9 0.8

z0: Tolerant, 9: Susceptible

y0: No shattering, 1: difficult, 5: Medium, 9:Easy to shattering

xShattering after 48hr at 40°C dry oven

콩 모자이크 바이러스병(SMV)에 대한 인위검정포장에서는 시험기간 3년 동안 괴저 증상은 없었고 강한 저항성을 보였다. 실내 유묘검정시험에서는 G5계통에서 상위엽에 모자이크 증상 을 나타내었고 G6H와 G7H계통에서는 접종엽에 반점이 나타났 으나 상위엽에는 이병증상이 없었다. 불마름병 저항성은 자연 발병 시험포장에서는 ‘1’, 유묘검정시험에서는 ‘6’으로 조사되 었다. 검은뿌리썩음병에 대한 저항성은 1.9%로 조사되었고, 종 자 병해는 갈반병이 약간 높았으나 자반병과 미이라병은 경미하 였다(Table 5).
Table 5.
The response of ‘Hanol’ to the diseases of soybean estimated from 2007 to 2009.
Table 5.
Cultivar Bacterial pustule (0-9)z
 Root rota (%) Soybean Mosaic Virus
 Percentages of damaged seed(%)a
Field Inoculationy Inoculationx
 Field (0-9) Purple seed stain Seed spot Phomopsis
G7H G6H G5
Hanol 1 6 1.9 -/M L/- L/- 1 0.9 2.1 0.8
Keunolkong 2 4 0.9 -/M -/M L/M 0 0.8 0.9 0.9

aRoot rot and damaged seed that were naturally infected in the field were estimated.

z0: Resistant, 9: Susceptible

y8ra strain

xReaction of inoculated leaf/upper leaves, - : No symptom, L: Local reaction, M: Mosaic

종실품질 및 가공적성
‘한올’의 조단백질 함량은 43.0%, 조지방 함량은 16.3%, 지방 산 조성 중에서 불포화지방산은 84.2%, 포화지방산은 15.8%를 차지하였다. 기능성 성분인 Isoflavone함량은 806 μg/g으로 생 육기간이 110일~120일 정도인 일반 장류용 콩보다 적은 수준이 었다(Table 6).
Table 6.
Major component of Seed in ‘Hanol’ estimated in 2009.
Table 6.
Cultivar Crude protein (%) Crude oil (%) Fatty acids (%)
 Isoflavone (µg/g)
Sat Unsat Daidzein Glycitein Genistein Total
Hanol 43.0 16.3 15.8 84.2 218 213 375 806
Keunolkong 42.1 17.0 16.3 83.7 241 171 499 911
‘한올’의 두부 및 비지수율은 각각 191%, 145%로 ‘큰올콩’과 비슷한 수준이었고, 두부의 색도는 ΔE 값이 3.1로 대조품종과 상당히 차이가 있었으며 두부의 물성에서 ‘큰올콩’보다 탄력성 은 낮았으나, 검성, 응집성, 경도는 높았다(Table 7). 메주수율과 청국장 수율은 각각 80%와 204%로 ‘큰올콩’보다 높았으며 발효정도와 풍취는 비슷하였다(Table 8).
Table 7.
Characteristics of tofu of ‘Hanol’ estimated in 2009.
Table 7.
Cultivar Yield (%) Bean Curd Residue (%) Color valuez
 Physical characteristics
L a b ΔE Elasticity Gum Cohesiveness Hardness (g/3.14mm2)
Hanol 191 145 86.4 -0.01 12.5 3.1 0.89 381 0.64 599
Keunolkong 196 151 85.1 0.9 15.2 1.08 317 0.62 509

zL : lightness, a : (-)Green~(+)Red, b : -(Blue)~(+)Yellow, ΔE : 3.0~6.0% (Appreciable difference)

Table 8.
Soybean malt and fermented soybean processability by ‘Hanol’.
Table 8.
Cultivar Soybean malt
 Fermented soybean
Degree of fermentation(1-5)z Yield(%) Scent (1-5) Degree of fermentation(1-5) Yield(%) Scent(1-5)
Hanol 4 80 4 3 204 4
Keunolkong 3 77 3 3 193 4

z1: worst, 2: bad, 3: fair, 4: good, 5: best

수량성
‘한올’은 2년간('05~'06) 실시한 생산력검정시험 결과 ha당 평균수량은 2.66톤으로 ‘큰올콩’은 2.16톤으로 조사되었다 (Table 9). 전국 6개 시험지에서 3년간(‘07~'09) 실시한 지역적 응시험 결과 ‘한올’은 수원을 제외한 모든 지역에서 10% 이상 증수한 것으로 나타났고 3년 평균수량은 ha당 2.04톤, ‘큰올콩’ 은 1.80톤이었다(Table 10).
Table 9.
Yield of ‘Hanol’ on yield trials carried out at Daegu.
Table 9.
Cultivar Yield (Ton/ha)
 Indexx
PYTz('05) AYTz('06) Mean
Hanol 2.87 2.44 2.66 123
Keunolkong 2.47 1.84 2.16 100

zPYT : Preliminary yield trial, AYT: Advanced yield trial.

xIndex : Mean yield of cultivar/Mean yield of Keunolkong × 100

Table 10.
Yield of ‘Hanol’ on the regional yield trials carried out at 6 locations
Table 10.
Location Hanol (Ton/ha)
 Keunolkong (Ton/ha)
2007 2008 2009 Mean Indexz 2007 2008 2009 Mean
Suwon 1.51 2.18 1.97 1.89 99 1.83 1.97 1.92 1.91
Daegu 1.76 2.04 - 1.90 111 1.69 1.73 - 1.71
Miryang - - 2.33 2.33 119 - - 1.95 1.95
Iksan 1.32 2.22 1.66 1.73 132 1.03 1.56 1.35 1.31
Jinju 2.07 3.04 2.60 2.57 113 1.81 2.77 2.26 2.28
Jeju - - 1.85 1.85 125 - - 1.48 1.48
Mean 1.67 2.37 2.08 2.04 114 1.59 2.01 1.79 1.80
t-value 1.42ns

zIndex = yield of Hanol / yield of Keunolkong × 100

nsNon significant.

재배상의 유의점
‘한올’의 적응지역은 전국 콩 재배지이며, 6월 상순에 파종하 면 남부지역은 9월 상순, 중부지역은 9월 중순에 수확되어 동계 작물인 마늘, 양파, 맥류 등과 작부체계가 가능하다. 조기 파종은 종실의 품위를 떨어뜨릴 우려가 있으므로 피해야 하고 수확이 지연되면 탈립이 잘 되고 병해립의 발생이 증가하므로 적기에 수확해야 한다.
‘한올’은 조숙 대립 다수성 콩 신품종육성을 목표로 조숙 대립 인 ‘SS91408’을 모본으로, 조숙인 ‘화엄풋콩’을 부본으로 1999 년에 교배한 것으로, 2000년부터 F1, F2 세대를 양성하고, F3부터 계통 전개하여 선발한 SS99408-2B-20-3-2 계통이다. 계통명은 ‘밀양184호’이며 2009년 12월 농작물 직무육성 신품종선정위 원회에서 국가목록등재품종으로 선정되어 ‘한올’로 명명하였다. ‘한올’은 유한신육형이며 엽형은 난형이고, 꽃색은 백색이다. 모용색은 회색이고, 협색은 담갈색, 종피색은 황색, 배꼽색은 옅은 갈색이며 종실모양은 구형이다. 개화와 성숙은 ‘큰올콩’보 다 다소 늦지만, 생육기간이 90일 내외인 단기성 특성을 잘 보이며 전국 평균 성숙기는 9월 14일이다.
경장은 39cm로 ‘큰올콩’보다 다소 크지만 도복에 강하며, 습해와 한발에는 중간 정도의 내성을 나타내며, 내탈립성은 약하 다. SMV 저항성은 포장에서 강하였고 유묘검정에서는 G5 계통 에서 모자이크 증상을 보였다. 종실의 조단백질 함량은 43.0%이 고 조지방 함량은 16.3% 수준이었다. ‘한올’의 종실 100립중은 26.4g으로 대립종이며, 전국 콩 재배지에서의 수량성은 ha당 2.04톤이었다.
본 연구는 농촌진흥청 연구사업(과제명: 콩 전통식품 가공적 성 신품종 육성, 과제번호: PJ01122504)의 지원에 의해 수행되 었다.
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Soybean Variety, ‘Hanol’ Adaptable for Double Cropping System with Early Maturing and Large Seed
Korean. J. Breed. Sci.. ;48(4):492-498.   Published online December 31, 2016
DOI: https://doi.org/10.9787/KJBS.2016.48.4.492
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Soybean Variety, ‘Hanol’ Adaptable for Double Cropping System with Early Maturing and Large Seed
Korean. J. Breed. Sci.. ;48(4):492-498.   Published online December 31, 2016
DOI: https://doi.org/10.9787/KJBS.2016.48.4.492
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Soybean Variety, ‘Hanol’ Adaptable for Double Cropping System with Early Maturing and Large Seed
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Fig. 1. Pedigree diagram of ‘Hanol’. a; PYT: Preliminary yield trial, b; AYT: Advanced yield trial, c; RYT: Regional yield trial.

Fig. 1.

Soybean Variety, ‘Hanol’ Adaptable for Double Cropping System with Early Maturing and Large Seed

Qualitative characteristics of ‘Hanol’

Cultivar Growth habit Leaflet shape Flower color Pubescence color Seed coat color Pod color Hilum color Seed shape
Hanol Determinate Oval White Gray Yellow Light brown Yellow Spherical
Keunolkong Determinate Oval White Gray Yellow Light brown Yellow Spherical

Quantitative characteristics of ‘Hanol’ determined by regional yield trial from 2007 to 2009.

Cultivar Flowering date Maturity date Stem height (cm) No. of nods No.of branch No. of pods per plant Weight per 100 seeds (g)
Hanol July 25 Sep. 14 39±5.2az 11±1.2a 2.1±0.89a 23±7.3a 26.4±2.31a
Keunolkong July 22 Sep. 9 34±4.7b 10±0.9a 2.0±0.71a 22±7.8a 27.9±2.46a

Value of characteristics ± Standard Error Same letters in the column are not significantly different by DMRT at 5%.

Maturing date and growing days of ‘Hanol’ on the regional yield trials carried out at six locations

Location Hanol (Month. Date)
 Keunolkong (Month. Date)
2007 2008 2009 Mean Growing Period (days) 2007 2008 2009 Mean Growing Period (days)
Suwon Sep.20 Sep.15 Sep.8 Sep.14 99 Sep.12 Sep.1 Sep.2 Sep.5 90
Daegu Sep.26 Sep.12 - Sep.19 99 Sep.14 Sep.12 - Sep.13 93
Miryang - - Sep.14 Sep.14 94 - - Sep.10 Sep.10 90
Iksan - Sep.22 Sep.18 Sep.20 86 - Sep.22 Sep.22 Sep.22 90
Jinju Sep.15 Sep.13 Sep.8 Sep.12 90 Sep.9 Sep.8 Sep.3 Sep.6 85
Jeju - - Sep.5 Sep.5 88 - - Sep.1 Sep.1 84
Mean Sep.20 Sep.15 Sep.14 Sep.14 94 Sep.11 Sep.10 Sep.7 Sep.9 89

The response of ‘Hanol’ to abiotic stress and insect estimated from 2007 to 2009.

Cultivar Lodging (0-9)z Water stress (0-9)z
 Shattering y
 Damaged seed for Pod borer (%)
Excess water Drought Field(0-9) Ratios by Oven test(%)xz
Hanol 0 4 4 9 9 0.7
Keunolkong 0 8 5 9 9 0.8

0: Tolerant, 9: Susceptible

0: No shattering, 1: difficult, 5: Medium, 9:Easy to shattering

Shattering after 48hr at 40°C dry oven

The response of ‘Hanol’ to the diseases of soybean estimated from 2007 to 2009.

Cultivar Bacterial pustule (0-9)z
 Root rota (%) Soybean Mosaic Virus
 Percentages of damaged seed(%)a
Field Inoculationy Inoculationx
 Field (0-9) Purple seed stain Seed spot Phomopsis
G7H G6H G5
Hanol 1 6 1.9 -/M L/- L/- 1 0.9 2.1 0.8
Keunolkong 2 4 0.9 -/M -/M L/M 0 0.8 0.9 0.9

Root rot and damaged seed that were naturally infected in the field were estimated.

0: Resistant, 9: Susceptible

8ra strain

Reaction of inoculated leaf/upper leaves, - : No symptom, L: Local reaction, M: Mosaic

Major component of Seed in ‘Hanol’ estimated in 2009.

Cultivar Crude protein (%) Crude oil (%) Fatty acids (%)
 Isoflavone (µg/g)
Sat Unsat Daidzein Glycitein Genistein Total
Hanol 43.0 16.3 15.8 84.2 218 213 375 806
Keunolkong 42.1 17.0 16.3 83.7 241 171 499 911

Characteristics of tofu of ‘Hanol’ estimated in 2009.

Cultivar Yield (%) Bean Curd Residue (%) Color valuez
 Physical characteristics
L a b ΔE Elasticity Gum Cohesiveness Hardness (g/3.14mm2)
Hanol 191 145 86.4 -0.01 12.5 3.1 0.89 381 0.64 599
Keunolkong 196 151 85.1 0.9 15.2 1.08 317 0.62 509

L : lightness, a : (-)Green~(+)Red, b : -(Blue)~(+)Yellow, ΔE : 3.0~6.0% (Appreciable difference)

Soybean malt and fermented soybean processability by ‘Hanol’.

Cultivar Soybean malt
 Fermented soybean
Degree of fermentation(1-5)z Yield(%) Scent (1-5) Degree of fermentation(1-5) Yield(%) Scent(1-5)
Hanol 4 80 4 3 204 4
Keunolkong 3 77 3 3 193 4

1: worst, 2: bad, 3: fair, 4: good, 5: best

Yield of ‘Hanol’ on yield trials carried out at Daegu.

Cultivar Yield (Ton/ha)
 Indexx
PYTz('05) AYTz('06) Mean
Hanol 2.87 2.44 2.66 123
Keunolkong 2.47 1.84 2.16 100

PYT : Preliminary yield trial, AYT: Advanced yield trial.

Index : Mean yield of cultivar/Mean yield of Keunolkong × 100

Yield of ‘Hanol’ on the regional yield trials carried out at 6 locations

Location Hanol (Ton/ha)
 Keunolkong (Ton/ha)
2007 2008 2009 Mean Indexz 2007 2008 2009 Mean
Suwon 1.51 2.18 1.97 1.89 99 1.83 1.97 1.92 1.91
Daegu 1.76 2.04 - 1.90 111 1.69 1.73 - 1.71
Miryang - - 2.33 2.33 119 - - 1.95 1.95
Iksan 1.32 2.22 1.66 1.73 132 1.03 1.56 1.35 1.31
Jinju 2.07 3.04 2.60 2.57 113 1.81 2.77 2.26 2.28
Jeju - - 1.85 1.85 125 - - 1.48 1.48
Mean 1.67 2.37 2.08 2.04 114 1.59 2.01 1.79 1.80
t-value 1.42ns

Index = yield of Hanol / yield of Keunolkong × 100

Non significant.

Table 1. Qualitative characteristics of ‘Hanol’
Table 2. Quantitative characteristics of ‘Hanol’ determined by regional yield trial from 2007 to 2009.

Value of characteristics ± Standard Error Same letters in the column are not significantly different by DMRT at 5%.

Table 3. Maturing date and growing days of ‘Hanol’ on the regional yield trials carried out at six locations
Table 4. The response of ‘Hanol’ to abiotic stress and insect estimated from 2007 to 2009.

0: Tolerant, 9: Susceptible

0: No shattering, 1: difficult, 5: Medium, 9:Easy to shattering

Shattering after 48hr at 40°C dry oven

Table 5. The response of ‘Hanol’ to the diseases of soybean estimated from 2007 to 2009.

Root rot and damaged seed that were naturally infected in the field were estimated.

0: Resistant, 9: Susceptible

8ra strain

Reaction of inoculated leaf/upper leaves, - : No symptom, L: Local reaction, M: Mosaic

Table 6. Major component of Seed in ‘Hanol’ estimated in 2009.
Table 7. Characteristics of tofu of ‘Hanol’ estimated in 2009.

L : lightness, a : (-)Green~(+)Red, b : -(Blue)~(+)Yellow, ΔE : 3.0~6.0% (Appreciable difference)

Table 8. Soybean malt and fermented soybean processability by ‘Hanol’.

1: worst, 2: bad, 3: fair, 4: good, 5: best

Table 9. Yield of ‘Hanol’ on yield trials carried out at Daegu.

PYT : Preliminary yield trial, AYT: Advanced yield trial.

Index : Mean yield of cultivar/Mean yield of Keunolkong × 100

Table 10. Yield of ‘Hanol’ on the regional yield trials carried out at 6 locations

Index = yield of Hanol / yield of Keunolkong × 100

Non significant.

Table 1.

Table 2.

Table 3.

Table 4.

Table 5.

Table 6.

Table 7.

Table 8.

Table 9.

Table 10.

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