Microspore-derived embryos (MDE) induction was investigated in 74 broccoli lines. The MDE induction was observedin only 18 broccoli lines. Especially, MDE was produced about 20 per petri dish in 08-8-8 and 08-8-33 lines. The influenceof MDE induction was investigated by flower structure and bud size (S, 0.1~0.2; M, 0.2~0.4; L, 0.4~0.6 mm) in 08-8-8 (highMDE induction line) and 08-8-10 (no MDE induction line) lines. There was no difference in structure of flower bud between08-8-8 and 08-8-10 lines, but M size flower bud has much longer stigma than petal in only 08-8-8 line. There was no observationof microspore at S size flower bud of 08-8-8 and 08-8-10 lines. The No. of MDE was correlated with the flower bud size.Microspore density of anther was much higher in the 08-8-8 than in the 08-8-10 line. Most of microspores in anther were atlate uninucleate stage in 08-8-8 (91.3%) and in 08-8-10 line (45.7%).
