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"Allelic variation"

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"Allelic variation"

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Evaluation of Gliadin and Storage Protein Activator (Spa) in Korean Wheat Cultivars
Ji-Eun Kim1, Young-Mi Yoon1,2, Seong Woo Cho1, Chon-Sik Kang2, Jong-Yeol Lee3, and Chul Soo Park1*
Korean. J. Breed. Sci. ;48(3):206-216.   Published online September 30, 2016
DOI: https://doi.org/10.9787/KJBS.2016.48.3.206
Genetic variations of γ-/ω-gliadin and Spa (storage protein activator) in 40 Korean wheat cultivars were evaluated to provide genetic information for improving end-use quality in wheat breeding programs. Korean wheat cultivars were classified into 13 patterns at the Gli-1 locus based on the allelic variation using A-PAGE (acidic-polyacrylamide gel electrophoresis). Seven, five, and six alleles were identified at Gli-A1, Gli-B1, and Gli-D1 loci, respectively. Allele-specific PCR markers for γ-gliadin corresponded to specific allele at Gli-1 loci on A-PAGE, which Gli-A1f, Gli-A1h and Gli-A1l alleles corresponded to GliA1.2, Gli-B1h and Gli-B1f alleles corresponded to GliB1.2 and Gli-D1f, Gli-D1m and Gli-D1o alleles corresponded to GliD1.1. DNA markers for γ-45 and γ-42 also corresponded to the γ-gliadin patterns around 40kDa on A-PAGE, except in Sukang, Ol and Joongmo2003. However, allelic specific PCR markers for ω5-gliadin did not correspond to that of A-PAGE. Three alleles were identified at Spa-A1 locus, whereas there was no variation at Spa-B1 and Spa-D1 loci.
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장수형 밀 계통의 특성 분석 II. 밀가루 및 관련 유전자 특성
Characterization of Korean Wheat Line with Long Spike. II. Flour Characteristics and Genetic Variations
Chon-Sik Kang, Jae-Han Son, Young-Keun Cheong, Kyeong-Hoon Kim, Yun-Hee Ko, Jong-Chul Park, Young-Jin Oh, Kyoung-Ho Kim, Bo-Kyoung Kim, Chul Soo Park
Korean. J. Breed. Sci. 2015;47(3):229-237.   Published online September 30, 2015
DOI: https://doi.org/10.9787/KJBS.2015.47.3.229

Molecular markers related to flour qualities of long spike wheat lines were evaluated. Ash, wet gluten and protein content of long spike wheat lines were higher than Keumkang(Korean wheat cv.) but SDS-sedimentation volume(SDSF) was lower. Particle size and lightness of flour of long spike wheat lines showed similar to Keumkang. The high positively correlation was presented between protein content and SDSF, wet gluten and particle size, respectively. However, negatively correlation was between protein content and lightness. Through the analysis of molecular markers, we identified the genotypes of four genes, Glu-A3c, Wx-A1a, Wx-B1a and Wx-D1a. These genes had same genotypes with Keumkang. Six genes, Glu-A1c, Glu-B1c, Glu-D1a, Glu-B3g, Pina-D1and Pinb-D1b, were presented in most of the lines. Two genes, Glu-A1 and Glu-B1b, were related to higher protein contents and wet gluten. The lines containing three genotypes, Glu-D1d, Glu-B3h and Pinb-D1b, had higher ash, protein contents, SDSF and wet gluten, and lightness was darker. Through the results, we expect that long spike type wheat lines are necessary to breed wheat having high yield productivity.

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장수형 밀의 계통 특성 분석 I. 농업 형질과 관련 유전자 특성
Characterization of Korean Wheat Line with Long Spike. I. Agronomic Traits and Genetic Variations
Jae-Han Son, Chon-Sik Kang, Young-Keun Cheong, Kyeong-Hoon Kim, Hag-Sin Kim, Jong-Chul Park, Kyoung-Ho Kim, Bo-Kyoung Kim, Chul Soo Park
Korean. J. Breed. Sci. 2015;47(3):219-228.   Published online September 30, 2015
DOI: https://doi.org/10.9787/KJBS.2015.47.3.219

One hundred sixty four wheat (Triticum aestivum L.) lines expressing the long spike were developed by the National Institute of Crop Science, Rural Development Administration. Six of 11 genes showed allelic variation among 164 lines and they were highly related to yield traits containing spike and kernel number, kernel length, 1000-kernel weight and grain yield. Analysis of allelic variation of each gene showed high correlation between allelic variation and agricultural characteristics. Coefficient of correlation between culm length and spike length was the highest 0.436. The heading date of the lines expressed Vrn-D1 allele was late and the lines expressed Vrn-D1 allele had longer spike length and higher number of kernel per spike than other lines. There was no significant difference in agronomic characteristics of analysis of Vrn-B1. Ppd-D1b was related to heading date and higher number of spike/m2. Ppd-D1a was corresponded to number of kernel per spike and 1000-kernel weight. Three haplotypes, Hap-L, Hap-6A-G and TaCwi-A1a, were related to 1000-kernel weight.

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