Flooding stress causes a significant reduction in soybean yield. The development of flood-tolerant cultivars is an effective way to minimize yield loss due to flooding. Information on candidate genes for flooding tolerance is useful for developing tolerant lines. The
objective
of this study was to identify potential candidate genes for flooding tolerance in soybean by integrating the results of a quantitative trait locus analysis and RNA sequencing. A total of 19 genes showed good amplification in capillary electrophoresis and were further analyzed through a reverse transcription quantitative polymerase chain reaction (qRT-PCR); two of these genes showed differential expression among tolerant and susceptible lines. The expression of Glyma.12g030900 and Glyma.10g050300 in leaf and root tissues, respectively, was higher in several tolerant lines than in the susceptible lines under flooding stress. The chlorophyll index of the tolerant lines was also consistently higher than that of the susceptible lines over two years, supporting the qRT-PCR results. This study provides useful information on flooding tolerance in soybeans.

A new peanut variety ‘Daan’(Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2014. This was developed from the cross between ‘Iksan 31’ with Virginia typed short stem and ‘Dakwang’ with Shinpung-typed larger grain. ‘Daan’ which is a Shinpung plant type had 13 branches per plant and its length of main stem was 44cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 34 pods per plant, 127 g of 100-seed-weight and 75% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 47.8% of crude oil and 28.3% of protein content. This variety showed resistant to early leaf spot and had more resistant to late leaf spot, stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years ‘Daan’ was more productive than reference variety by 16% with 5.00 MT/ha for grain production.

A new peanut variety ‘Danwon’(Arachis hypogaea ssp. fastigiata L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2013. This was developed from the cross between ‘YG75’ with Shinpung type and ‘SP9617’ with larger grain. ‘Danwon’ is the first Shinpung typed short stem variety ever developed in Korea. It has 13 branches per plant and its length of main stem was 32 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 40 pods per plant, 91 g of 100-seed-weight and 77% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 48.7% of crude oil and 26.9% of protein content. This variety showed more resistant to early and late leaf spot, web blotch, stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years ‘Danwon’ was more productive than reference variety by 6% with 4.52 MT/ha for grain production.

A new peanut variety ‘K-Ol’(Arachis hypogaea ssp. fastigiata L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2013. This was developed from a cross between ‘Baekseon’ with Shinpung type and ‘F435-5’ with high oleic acid. ‘K-Ol’ is the first high oleate variety in Korean and has short stem and Shinpung plant type. It has 10 branches per plant and its length of main stem was 43 cm. Each pod has two grains with brown testa and short ellipse-shaped kernel. Its yield components showed 46 pods per plant, 74 g of 100-seed-weight and 76% of pod shelling ratio in the regional yield trials (RYT). Seed contains 26.0% protein and 50.7% crude oil with a typical fatty acid composition of 82.9% oleate and 2.7% linoleate. This variety showed more resistant to late leaf spot, web blotch, stem rot and lodging, compared to reference variety ‘Daekwang’. In the regional yield trials for 2 years ‘K-Ol’ showed 4.34 MT/ha for grain yield by 99% of production level of reference variety.

A new peanut variety ‘Huaseon’ (Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2013. This was developed from the cross between ‘Iksan 31’ with Virginia typed short stem and ‘SP9614’ with Shinpung-typed larger grain. ‘Huaseon’ which is a Shinpung plant type had 10 branches per plant and its length of main stem was 41 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 45 pods per plant, 84 g of 100-seed-weight and 79% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 47.3% of crude oil and 27.6% of protein content. This variety showed resistant to early leaf spot and had more resistant to stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years ‘Huaseon’ was more productive than reference variety by 12% with 4.78 MT/ha for grain production.

A new peanut variety ‘Sinpalkwang’ (Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2012. This was developed from the cross between line ‘HP299’, crossed high yielding variety ‘Palkwang’ and short stem cultivar ‘PI156649’, and ‘Palkwang’. ‘Sinpalkwang’ which is a Virginia plant type had 25 branches per plant and its length of main stem was 41 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 45 pods per plant, 92 g of 100-seed-weight and 79% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 45.4% of crude oil and 29.2% of protein content. This variety also showed more resistant to web blotch, compared with reference variety. In the regional yield trials for 3 years ‘Sinpalkwang’ was more productive than reference variety by 28% with 5.4 MT/ha for grain production.

A new peanut variety ‘Ami’ (Arachis hypogaea ssp. fastigiata.) was bred at the Department of Functional Crop, NICS, in Milyang in 2012. This was developed by pedigree method after the cross between the short stem cultivar ‘Satonoka’ and the disease resistant cultivar ‘Milyang16’. ‘Ami’ which is Shinpung plant type had 10 branches per plant and its length of main stem was 50 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel, and its 100 seed weight was 84 g in the regional yield trials (RYT). ‘Ami’ showed 50.5% of crude oil and 60.5% of oleic acid composition in dry seed, and had 24.9 mg/g of sucrose and 4.5 mg/g of tannin content in fresh peanut. In the regional yield trials, ‘Ami’ was more productive than reference variety by 7% with 10.10 MT/ha for fresh pod production and by 14% with 4.51 MT/ha for dry grain production.

A new peanut variety ‘Sangan’ (Arachis hypogaea ssp. fastigiata L.) was bred at the Department of Functional Crop, National Institute of Crop Science (NICS), in Milyang 2011. This was developed from the cross between high quality cultivar ‘Palkwang’ and fewer branched germplasm ‘Chinese collect. 1’. ‘Sangan’ which is Shinpung plant type had 10 branches per plant and its length of main stem was 43 cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel, and 100 seed weight was 96 g in the regional yield trials (RYT). Seed quality showed 48.5% of crude oil and 29.1% of protein content. This variety also showed more resistant to late leaf spot and web blotch, compared with reference cultivar. In the regional yield trials ‘Sangan’ was more productive than reference cultivar by 15% with 4.87 MT/ha for grain production.

A new peanut variety ‘Jaseon’ (Arachis hypogaea ssp. fastigiata.) was developed by the Department of Functional Crop, NICS, in Milyang in 2011. This was developed from the cross between the large grain cultivar ‘YG60’ and the short stem cultivar ‘YG55’. ‘Jaseon’, which is a Shinpung plant type, has 11 branch number and 43 cm in main stem length. Each pod has two grains with brown testa and long-ellipse shaped large kernel and its 100 seed weight was 98 g in the regional yield trials (RYT). ‘Jaseon’ showed 43.2% of crude oil and 43.0% of oleic acid composition in dry peanut, and had 25.0 mg/g of Sucrose and 4.4 mg/g of tannin content in fresh peanut. In the regional yield trials, ‘Jaseon’ was outyielded by 15% with 10.21 MT/ha for fresh pod and by 11% with 4.37 MT/ha for dry grain, compared those of check variety.

A soybean cultivar for sprout, ‘Joyang 1’ was developed at the National Institute of Crop Science (NICS) in 2010. ‘Joyang 1’ (Iksan67) was released by pedigree selection from the cross between high yielding ‘Pungsannamulkong’ and ‘Suwon187’ tolerant to lodging and disease. It has purple flower, lanceolate leaf, grey pubescence and small yellow seed. Weight of hundred seed is 11.6gram. Maturing date of ‘Joyang 1’ is ten days earlier than check cultivar, ‘Pungsannamulkong’. ‘Joyang 1’ is resistant to bacterial pustule and shattering. It have similar tolerance to root rot and seed spot to check cultivar. It shows high germination ratio, less abnormal germination and hard seed. Sprout yield also 11 percent higher than check cultivar. The average seed yield of ‘Joyang 1’ was 3.03 ton per hectare in the regional yield trials (RYT) carried out for three years from 2008 to 2010 which was 3 percent higher than the check cultivar. The number of breeder’s right is ‘4900’.