Male sterility is used to mass-produce F₁ hybrid seeds in pepper (Capsicum annuum L.). In particular, the development of genetic male sterility (GMS)-linked molecular markers may play a crucial role in hybrid breeding of pepper. To date, approximately 20 GMS genes have been identified in pepper. Among these, several molecular markers for the ms₃ gene have been developed in previous studies; however, they are not completely linked and thus have limitations for use in selection. Therefore, in the present study, we aimed to develop molecular markers for ms₃ selection using single-nucleotide polymorphism (SNP)-based high-resolution melting (HRM) analysis. Chi-square test was conducted using three F₂ segregating populations, and the results confirmed a 3:1 segregation ratio between male-fertile and male-sterile plants. A total of 128 primer sets were designed by selecting SNPs near the ms₃ gene, and 25 HRM markers were successfully developed. Using 420 individuals from the F₂ segregating population ‘GMS3,’ a high-density genetic linkage map of pepper chromosome 1 was constructed, with eight HRM markers found to be co-segregated with the ms₃ gene. Subsequent experiments using various plant materials validated these eight markers, ultimately identifying two HRM markers, HRM119655681 and HRM135273656, for the final selection. These two markers showed co-segregation between the phenotype and genotype of ms₃ across all plant materials used in the study. The markers developed in this study are expected to be effective for maternal line development and large-scale F₁ hybrid seed production using ms₃ in pepper.

‘Miwoo’ is a mid-late maturing, high-biomass-yielding rice (Oryza sativa L.) cultivar with high feed value and resistance to multiple diseases and insect pests. It was developed for use as whole crop silage (WCS) from a cross between ‘Suweon519’, a line with high biomass yield and multiple disease resistance, and ‘Suweon518’, a medium flowering, high yielding Tong-il type line, which is resistant to blast (BL), bacterial blight (BB) (race K1), rice stripe virus (RSV) and brown planthopper (BPH). In the central plain region of Suwon, ‘Miwoo’ cultivar exhibited a growth period of approximately 122 days from seeding to heading, culm length of 99 cm, panicle length of 28 cm, 12 panicles per hill, 143 spikelets per panicle, and 1,000-grain weight of 25.9 g as brown rice. This mid-late-flowering rice variety was resistant to lodging during the yellowing ripening stage, an optimal period for harvesting. In addition, ‘Miwoo’ was strongly resistant to leaf and neck blast, BB (race K1, K2, K3, K3a), RSV, BPH and small BPH. Its average dry matter yield over 3 years reached 19.9 MT/ha, which is 36% higher than that of ‘Nokyang’. ‘Miwoo’ is a high-amylose cultivar with 70.7% total digestible nutrients (similar to that of Nokyang) and grows well on the central and southern plains of the Korean Peninsula. It should be harvested 30 days after heading to improve its feed value and digestion rate in livestock. (Registration No. 7683)

‘Jowoo’, an early maturing, high biomass yielding rice (Oryza sativa L.) cultivar with high feed value and multiple disease-insect resistance, was developed for winter forage crop-whole crop silage (WCS) rice double cropping. It was derived from a cross between a high biomass and multiple disease resistant line ‘Suweon519’ and an early flowering, high yielding Tong-il type variety ‘Gaya’ that harbored Bph3 and BPH26, conferring brown planthopper (Bph) broad resistance. This cultivar had a growth period of approximately 106 days from seeding to heading, 97 cm culm length, 24 cm panicle length, 12 panicles per hill, 120 spikelets per panicle, and 1,000-grain weight of 22.1 g as brown rice in the central plain region of Suwon. This early flowering rice variety was tolerant to premature germination and cold stresses, especially showed higher grain fertility than ‘Nokyang’ at a cold-water (17℃) irrigated screening nursery. Further, ‘Jowoo’ exhibited high lodging resistance in the yellowing ripening stage, an optimal period for harvest. In addition, ‘Jowoo’ was resistant to leaf and neck blast, bacterial blight (race K1, K2, K3, K3a), rice stripe virus, and small Bph and was also moderately resistant to Bph. Its average dry matter yield for three years reached 18.2 MT/ha, the highest among early flowering rice varieties and 19% higher than that of ‘Nokyang’. This cultivar had 72.2% total digestible nutrients, which is also higher than that of ‘Nokyang’. In the Korean peninsula, ‘Jowoo’ grows well in the central and southern plains and can be harvested 30 days after heading. This helps improve its feeding value and digestion rate in livestock (Grant No. 8133).

‘Cheongwoo’, a late maturing, high biomass-yielding rice (Oryza sativa L.) cultivar with high feed value and multiple disease and insect resistance, was developed for whole crop silage (WCS) use. It was derived from a cross between a high biomass and multiple disease resistant line ‘Suweon519’ and early flowering, high-yielding Tong-il type cultivar ‘Gaya’, which exhibited a brown planthopper (BPH) broad resistance conferred by Bph3 and BPH26. The cultivar was characterized by growth period from seeding to heading of about 130 days, culm length of 87 cm, panicle length of 29 cm, 12 panicles per hill, 135 spikelets per panicle, and 1,000-grain weight of brown rice of 21.4 g in the central plain region, Suwon. This long-leaved green WCS rice variety was sensitive to cold stress, similar to ‘Nokyang’, but resistant to premature germination, and germinated well under low temperature conditions. Furthermore, ‘Cheongwoo’ exhibited high lodging resistance at the yellowing stage, the optimal period for harvesting in the field. Additionally, ‘Cheongwoo’ was resistant to leaf and neck blast, bacterial blight (races K1, K2, K3, K3a), rice stripe virus, BPH, and small BPH. Its average dry matter yield for three years reached 20.6 MT/ha, 38% higher than that of ‘Nokyang’. The content of crude protein (5.32%) and total digestible nutrients (71.2%) was higher than that in ‘Nokyang’. On the Korean Peninsula, ‘Cheongwoo’ grows well in central and southern plains, and should be optimally harvested between 15 and 30 days after heading to improve its feeding value and digestion rate in livestock (Grant No. 7662).

‘Haedamssal’ is an early maturing and rice stripe virus disease-resistant cultivar adaptable for early-transplanting cultivation that was developed by the rice breeding team of the Department of Southern Crop, NICS, RDA, in 2014. This cultivar was derived from the cross YR25869 (YR21247-B-B-B-49-1/Sasanishiki BL4//Koshihikari) and YR25868 (Unkwang//YR21247-B-B-B-49-1/Sasanishiki BL4) made in the 2005/2006 winter season and was advanced to the F₅ generation by a bulk breeding method using rapid generation advance. To incorporate rice stripe virus resistance, marker-assisted selection on the RSV gene was conducted in 3-way and 6-way cross F₁ generation using the tightly linked marker RM6897. From testing in the replicated yield trial in 2011, a promising line YR26258-B-B-B-33-3 was selected and it was designated as ‘Milyang276’. A local adaptability test of ‘Milyang276’ was performed at three locations from 2012 to 2014 and it was named as ‘Haedamssal’, which was a good eating quality variety. The culm length was 67 cm in yield trials, which was 4 cm shorter than ‘Jopyeong’. The number of spikelets per panicle was lower than ‘Jopyeong’, whereas the number of tillers per hill was higher. This variety was resistant to RSV disease, bacterial blight, and leaf blast disease. The milled rice yield of ‘Haedamssal’ was 5.48 MT per ha at the early transplanting in the local adaptability test. ‘Haedamssal’ is well adapted to early transplanting cultivation in the southern plain area (Registration No. 6811).

Hexaploid wheat (common wheat/bread wheat) is one of the most important cereal crops in the world and a model for research of an allopolyploid plant with a large, highly repetitive genome. In the heritability of agronomic traits, variation in gene presence/absence plays an important role. However, there have been relatively few studies on the variation in gene presence/absence in crop species, including common wheat. Recently, a reference genome sequence of common wheat has been fully annotated and published. In addition, advanced next-generation sequencing (NGS) technology provides high quality genome sequences with continually decreasing NGS prices, thereby dawning full-scale wheat functional genomic studies in other crops as well as common wheat, in spite of their large and complex genomes. In this review, we provide information about the available tools and methodologies for wheat functional genomics research supported by NGS technology. The use of the NGS and functional genomics technology is expected to be a powerful strategy to select elite lines for a number of germplasms.

‘Yeongwoo’, a mid-late flowering, high dry matter yielding rice (Oryza sativa L.) cultivar with high feed value and multiple disease-insect resistance, was developed for whole crop silage (WCS) use. It was derived from a cross between a high biomass and brown planthopper (Bph) resistant ‘SR25848-C99-1-2-1’ and high yielding Tong-il type, ‘Yongmun’ which had diseases resistance. This cultivar had about 117 days growth period from seeding to heading, 99 cm culm length, 26 cm panicle length, 11 panicles per hill, 125 spikelets per panicle, and 1,000-grain weight of 23.6 g as brown rice in the central plain region, Suwon. This wide and long leafy WCS rice variety was weak to cold stresses, similarly to ‘Nokyang’ but was resistant to lodging in the field, strong to viviparous germination, and good under low temperature germination. In addition, ‘Yeongwoo’ was resistant to leaf and neck blast, bacterial blight (race K1, K2, K3, K3a), rice stripe virus, Bph, and small Bph. Its average dry matter yield for three years reached 20.0 MT/ha, 32% higher than that of ‘Nokyang’. This cultivar had 5.3% crude protein and 71.7% total digestible nutrients, which was slightly lower than that of ‘Nokyang’. In the Korean peninsula, ‘Yeongwoo’ grew well in the central and southern plain and was could be harvested between 15 to 30 days after heading to improve the feeding value and digestion rate of livestock (Grant No. 6800).

Kenaf (Hibiscus cannabinus L.) is an annual herbaceous crop of the Malvaceae family. Recently, kenaf is being used in many diversified applications such as pulp, animal feed, bioplastics and cellulosic biofuel etc. A new cultivar 'Jangdae' was developed by mutation breeding using irradiated with 300 Gy gamma-rays. Jangdae has a few distinguishable characteristics such as early flowering, high seed yield and palmate leaf, compared to wild type (Jinju). The fresh matter (FM) and dry matter (DM) yield of Jangdae are similar to those of Jinju, their seed yield (833.3 kg/ha) is approximately 4 times higher than that of Jinju. In addition, the FM and DM yield of Jangdae are 40% higher than those of early-maturing cultivar (C11). We performed the nutritive value of four kenaf cultivars (Jangdae, Jinju, C11 and Hongma300) at 100 day after seeding. The crude protein content of Jangdae, Jinju, C11 and Honma300 were 10.5, 11.0, 9.4 and 10.6%, respectively. The crude fiber, neutral detergent fiber and acid detergent fiber contents were no significant difference among the cultivars. Jangdae, which afforded both a high biomass and seed yield in South Korea, may be useful as potential source of feed and industrial materials.

The amylose contents of rice determine eating quality which is one of the major traits in rice breeding program. To identify the low-amylose gene of the japonica rice cultivar Baegjinju, genetic analysis was conducted using 200 F₂ population derived from a cross between the japonica cultivars, Saeilmi and Baegjinju. Individual F₂ plants were classified as wild type (translucent grain) and mutant type (dull grain) based on the grain appearance of brown rice. Two hundred F₂ plants were segregated into 155 wild type plants and 45 mutant type plants, which fit the 3:1 ratio (x² = 0.667, df = 1, p = 0.414) and this result indicated the low-amylose gene of Baegjinju is a single recessive gene which controls the amylose contents. Linkage analysis was conducted to localize the low-amylose gene of Baegjinju and fine mapped within an 800-kb interval between 17.5 to 18.8Mb on short arm of chromosome 10. Co-segregated SSR marker, RM25648 was developed and it could be useful for marker-assisted selection and determination of the genetic resource related with amylose contents in rice breeding.