1 전남농업기술원 식량작물연구소
1 Crop Research Division, JARES, Naju 58213, Korea
2 전남농업기술원 원예연구소
2 Horticulture Research Division, JARES, Naju 58213, Korea
3 국립식량과학원 중부작물부
3 Department of Central Area Crop Science, NICS, RDA, Suwon 16429, Korea
4 국립식량과학원 남부작물부
4 Department of Southern Area Crop Science, NICS, RDA, Miryang 50424, Korea
© Korean Society of Breeding Science. All rights reserved.
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| Items | Conditions |
|---|---|
|
|
|
| Mobile phase | A(H2O : EtOAC = 92 : 8) : B(MeOH) = 85 : 15 |
| Flow rate | 1 mL/min |
| Detector | Photo diode array 254nm |
| Column Type | SunFire C18 5㎛, 4.6×150㎜ |
| Oven temperature | 40℃ |
| SSR maker | CEDG006 | CEDG008 | CEDG037 | CEDG071 | CEDG141 | CEDG147 |
|---|---|---|---|---|---|---|
| Cultivar | ||||||
|
|
||||||
| Sanpo | az | c | a | a | a | b |
| Owool | a | a | a | b | a | b |
| Cultivar | 1st flowering date | 1st maturing date | Stem length (cm) | No. of pod per plant | No. of seed per pod | 1,000-seed weight (g) |
|---|---|---|---|---|---|---|
|
|
||||||
| Sanpo | July 30 | Aug. 18 | 71* | 21 ns | 11.9 ns | 48 ns |
| Owool | July 28 | Aug. 17 | 66 | 20 | 11.7 | 48 |
| Cultivar | Mungbean mottle virus (0~9)z | Cercospora leaf spot (0~9) | Powdery mildew (0~9) | Lodging (1~9)y |
|---|---|---|---|---|
|
|
||||
| Sanpo | 0.8 | 1.0 | 0.0 | 2 |
| Owool | 1.3 | 1.5 | 0.1 | 4 |
| Cultivar | Nutritionals (%) | Flavonoids (mg/g) | ||
|---|---|---|---|---|
|
|
|
|||
| Starch | Crude protein | Vitexin | Isovitexin | |
|
|
||||
| Sanpo | 49.5±0.98 | 22.1±0.15 | 9.7±0.13 | 9.8±0.14 |
| Owool | 50.7±1.12 | 22.3±0.17 | 9.5±0.12 | 9.5±0.11 |
| Cultivar | Year | Hypocotyl length (㎝) | Root length (㎝) | Hard seed rate (%) | Sprout yield (%) | index (A/B) |
|---|---|---|---|---|---|---|
|
|
||||||
| Sanpo (A) | 2010 | 9.4 | 7.8 | 1.5 | 913 | 122 |
| 2011 | 6.8 | 7.4 | 5.6 | 622 | 97 | |
| 2012 | 5.4 | 7.4 | 0.1 | 944 | 110 | |
|
|
||||||
| Mean | 7.2 ns | 7.5 ns | 2.4 ns | 826 ns | 110 | |
|
|
||||||
| Owool (B) | 2010 | 7.9 | 7.5 | 4.5 | 750 | 100 |
| 2011 | 7.0 | 7.7 | 4.2 | 639 | 100 | |
| 2012 | 4.3 | 7.9 | 0.8 | 859 | 100 | |
|
|
||||||
| Mean | 6.4 | 7.7 | 3.2 | 749 | 100 | |
High performance liquid chromatography (HPLC) conditions for analysis of vitexin and isovitexin.
| Items | Conditions |
|---|---|
|
|
|
| Mobile phase | A(H2O : EtOAC = 92 : 8) : B(MeOH) = 85 : 15 |
| Flow rate | 1 mL/min |
| Detector | Photo diode array 254nm |
| Column Type | SunFire C18 5㎛, 4.6×150㎜ |
| Oven temperature | 40℃ |
Inherent characteristics of a new mungbean cultivar ‘Sanpo’.
| Cultivar | Growth habit | Leaflet shape | Hypocotyl color | Corolla color | Pod | Seed | ||||
|---|---|---|---|---|---|---|---|---|---|---|
|
|
|
|||||||||
| Color at maturity | Shattering | Curvature | Coat color | Surface luster | Shape | |||||
|
|
||||||||||
| Sanpo | Erect | Heart | Green | Light yellow | Black | Moderate | Straight | Green | Dull | Angular drum |
| Owool | Erect | Heart | Green | Light yellow | Black | Moderate | Straight | Green | Dull | Angular drum |
Analysis of SSR maker of a new mungbean cultivar ‘Sanpo’.
| SSR maker | CEDG006 | CEDG008 | CEDG037 | CEDG071 | CEDG141 | CEDG147 |
|---|---|---|---|---|---|---|
| Cultivar | ||||||
|
|
||||||
| Sanpo | a |
c | a | a | a | b |
| Owool | a | a | a | b | a | b |
zRelative size of allele at each SSR maker: a < b < c.
Agronomic characteristics of a new mungbean cultivar ‘Sanpo’ determined by regional yield trial from 2010 to 2012.
| Cultivar | 1st flowering date | 1st maturing date | Stem length (cm) | No. of pod per plant | No. of seed per pod | 1,000-seed weight (g) |
|---|---|---|---|---|---|---|
|
|
||||||
| Sanpo | July 30 | Aug. 18 | 71 |
21 |
11.9 |
48 |
| Owool | July 28 | Aug. 17 | 66 | 20 | 11.7 | 48 |
nsNot significant in the t-test, while
*Significant at P<0.05.
Field resistance to diseases and lodging of a new mungbean cultivar ‘Sanpo’ determined by regional yield trial from 2010 to 2012.
| Cultivar | Mungbean mottle virus (0~9) |
Cercospora leaf spot (0~9) | Powdery mildew (0~9) | Lodging (1~9) |
|---|---|---|---|---|
|
|
||||
| Sanpo | 0.8 | 1.0 | 0.0 | 2 |
| Owool | 1.3 | 1.5 | 0.1 | 4 |
z; 0: Resistant, 9: Susceptble
y; 1: Resistant, 9: Susceptble
Contents of nutritional and flavoniod of a new mungbean cultivar ‘Sanpo’.
| Cultivar | Nutritionals (%) | Flavonoids (mg/g) | ||
|---|---|---|---|---|
|
|
|
|||
| Starch | Crude protein | Vitexin | Isovitexin | |
|
|
||||
| Sanpo | 49.5±0.98 | 22.1±0.15 | 9.7±0.13 | 9.8±0.14 |
| Owool | 50.7±1.12 | 22.3±0.17 | 9.5±0.12 | 9.5±0.11 |
Sprout characteristics and yield a new mungbean cultivar ‘Sanpo’.
| Cultivar | Year | Hypocotyl length (㎝) | Root length (㎝) | Hard seed rate (%) | Sprout yield (%) | index (A/B) |
|---|---|---|---|---|---|---|
|
|
||||||
| Sanpo (A) | 2010 | 9.4 | 7.8 | 1.5 | 913 | 122 |
| 2011 | 6.8 | 7.4 | 5.6 | 622 | 97 | |
| 2012 | 5.4 | 7.4 | 0.1 | 944 | 110 | |
| |
||||||
| Mean | 7.2 |
7.5 |
2.4 |
826 |
110 | |
| |
||||||
| Owool (B) | 2010 | 7.9 | 7.5 | 4.5 | 750 | 100 |
| 2011 | 7.0 | 7.7 | 4.2 | 639 | 100 | |
| 2012 | 4.3 | 7.9 | 0.8 | 859 | 100 | |
| |
||||||
| Mean | 6.4 | 7.7 | 3.2 | 749 | 100 | |
ns, Not significant in the t-test.
Yield of a new mungbean cultivar ‘Sanpo’ determined by regional yield trial from 2010 to 2012.
| Location | Sanpo (MT/ha, A) | Owool (MT/ha, B) | Index (A/B) | ||||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|
||||||||
| 2010 | 2011 | 2012 | Mean | 2010 | 2011 | 2012 | Mean | ||
|
|
|||||||||
| Suwon (NICS) | 1.79 | 1.81 | 1.37 | 1.66 | 1.71 | 1.81 | 1.18 | 1.57 | 106 |
| Miryang (NICS) | 1.40 | 1.60 | 1.96 | 1.65 | 1.35 | 1.52 | 1.57 | 1.48 | 112 |
| Naju (JARES) | 2.14 | 1.09 | 1.43 | 1.55 | 1.59 | 0.83 | 1.24 | 1.22 | 127 |
| |
|||||||||
| Mean | 1.78 | 1.50 | 1.59 | 1.62 ns | 1.55 | 1.39 | 1.33 | 1.42 | 114 |
nsNot significant in the t-test.
Relative size of allele at each SSR maker: a < b < c.
Not significant in the t-test, while
Significant at P<0.05.
; 0: Resistant, 9: Susceptble
; 1: Resistant, 9: Susceptble
, Not significant in the t-test.
Not significant in the t-test.