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New Cultivar Developed

중만생 저아밀로스 가공용 벼 ‘미호’(밀양300호)

이지윤1, 이나금2, 송유천1, 이종희1, 조수민1, 권영호1, 이소명1, 강주원1, 박동수1, 조준현1,*

‘Miho’ (Milyang300), a Mid to Late Low-Amylose Variety of Processed Rice

Korean Journal of Breeding Science 2021;53(3):295-304.
Published online: September 1, 2021

1농촌진흥청 국립식량과학원

2리치코리아

1National Institute of Crop Science, RDA, Wanju, 55365, Republic of Korea

2RICHS.COM/RICHSKOREA. Co. Kr. 37, Dalseong 2-4, Daegu, 43013, Republic of Korea

*Corresponding Author (E-mail: hy4779@korea.kr, Tel: +82-55-350-1169, Fax: +82-55-352-3059)
• Received: May 18, 2021   • Revised: May 21, 2021   • Accepted: May 31, 2021

Copyright © 2021 by the Korean Society of Breeding Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Improved Processing Quality in a tongil-type Waxy Rice Cultivar Associated with a Rare granule-bound starch synthase I Allele
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    Korean Journal of Breeding Science.2026; 58(2): 123.     CrossRef
  • ‘Dapum’, a Late-Maturing Rice Cultivar with Low Amylose Content in the Chungnam Plain Area, Korea
    Giwon Cho, Gyucheol Kim, Chongtae Chung, Tugsang Yun, Yoetae Yun
    Korean Journal of Breeding Science.2024; 56(4): 513.     CrossRef

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‘Miho’ (Milyang300), a Mid to Late Low-Amylose Variety of Processed Rice
Korean. J. Breed. Sci.. 2021;53(3):295-304.   Published online September 1, 2021
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‘Miho’ (Milyang300), a Mid to Late Low-Amylose Variety of Processed Rice
Korean. J. Breed. Sci.. 2021;53(3):295-304.   Published online September 1, 2021
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‘Miho’ (Milyang300), a Mid to Late Low-Amylose Variety of Processed Rice
Image Image Image
Fig. 1 Pedigree diagram of ‘Miho’.
Fig. 2 Genealogical diagram of ‘Miho’.
Fig. 3 Field photo at maturing stage of ‘Miho’ and grain appearance (milled rice) of ‘Miho(left)’ and ‘Nampyeong (right)’.
‘Miho’ (Milyang300), a Mid to Late Low-Amylose Variety of Processed Rice

Major agronomic traits and yield components of ‘Miho’. (LAT: '14~'16)

Variety Heading date
(m.d)
Culm
length
(cm)
Panicle
length
(cm)
No. of
panicles per
hill
No. of
Spikelets per panicle
Ratio of
ripened grain
(%)
Brown rice
1000 grains
weight (g)
Miho 8.21** 67** 19ns 16ns 95ns 90.2ns 22.7**
Nampyeong 8.10 74 20 16 102 86.9 21.3

Viviparous germination and cold tolerance of ‘Miho’. (LAT: '14~'16)

Variety VVGz (25℃ at 40 DAF) Cold tolerancey
Heading delay
(days)
Grain fertility
(%)
PA (1-9)x
at maturing stage at seedling stage
Miho 36.9** 14ns 29.7ns 7ns 4ns
Nampyeong 8.3 9 32.0 6 3

Response to leaf and neck blast disease of ‘Miho’.

(LAT: '14~'16)

Variety Leaf blast diseasez Neck blast disease
No. of tested sites Ave. Rate of diseased panicle (%)
R
(0~3)
M
(4~6)
S
(7~9)
Iksan Miryang Icheon Jinju
Miho 5 6 1 4.6 0.0 2.0 0.1 0.0
Nampyeong 4 8 0 4.3 0.2 0.0 0.0 0.1

Response to bacterial blight, virus diseases and insect pests of ‘Miho’.

(LAT: '14~'16)

Variety Bacterial blight Virus diseasez Insect pestsy
K1 K2 K3 K3a RSV RDV RBSDV BPH SBPH
Miho Rx R R S R S S S S
Nampyeong R S S S R M S S S

Grain shape of rough and brown rice of ‘Miho’.

(LAT: '14~'16)

Variety Rough rice (mm) Brown rice (mm)
Length
(L)
Width
(W)
Thickness
(T)
Ratio of
L/W
Length
(L)
Width
(W)
Thickness
(T)
Ratio of
L/W
Miho 7.29ns 3.35ns 2.29ns 2.18ns 4.85* 2.90ns 2.02ns 1.67ns
Nampyeong 7.02 3.27 2.14 2.14 4.97 2.90 1.93 1.71

Physiochemical properties of ‘Miho’.

(LAT: '14~'16)

Variety Translucency
(1-9)
Amylose
(%)
Protein
(%)
1.4% KOH
Alkali digestion value (1-7)
Endosperm
Core/belly
(0-9)
Miho 5 11.2* 6.0ns 6.0 dull
Nampyeong 1 18.9 5.9 6.4 0/0

Rapid viscosity analysis of ‘Miho’.

Varieties Peak T.
(min)
Viscosity (RVU) Pasting Temp (℃)
Peak Hot paste Cold paste Breakdown Setback
Baekogchal 3.29±0.04d1) 110.94±7.63c 27.33±2.96d 38.47±2.54d 83.61±4.68bc 11.14±0.76d 66.47±1.29a
Baegjinju 5.49±0.04c 166.58±7.29b 62.42±3.33c 95.33±3.10c 104.17±4.51a 32.92±0.46c 68.10±2.55a
Miho 5.69±0.04b 165.36±0.57b 78.36±1.26b 122.53±0.97b 87.00±1.76b 44.17±0.30b 67.10±1.31a
Saeilmi 6.22±0.04a 214.53±3.54a 135.67±4.84a 224.61±4.16a 78.86±2.30c 88.94±1.55a 68.73±0.59a

Water absorption ratio (%) of ‘Miho’ based on soaking time.

Soaking time
(h)
Varieties
Baekogchal Baegjinju Miho Saeilmi
2 35.36±0.82a) 32.93±1.98a 33.23±0.56a 29.07±0.20a
6 34.36±1.17a 31.41±1.02a 32.47±1.70a 27.68±1.04a
24 34.18±1.48a 31.43±1.62a 31.05±1.36a 25.71±0.80b

Textural characteristics of cooked rice of ‘Miho’ based on storage time at 3℃.

Storage time (h) Varieties
Baekogchal Baegjinju Miho Saeilmi
Hardness
(N)
0 18.00 ±3.23b) 18.63 ±1.95b 17.95 ±3.61b 19.32 ±1.60b
6 22.52 ±4.15a 23.99 ±2.12a 27.52 ±2.34a 23.00 ±2.78a
24 22.96 ±2.94a 22.62 ±2.03a 25.91 ±3.31a 22.13 ±2.68a
48 17.95 ±4.27b 22.59 ±4.97a 28.53 ±3.95a 22.63 ±1.71a
Springiness 0 0.64 ±0.06b 0.46 ±0.05b 0.42 ±0.05b 0.38 ±0.06b
6 0.71 ±0.03a 0.55 ±0.05a 0.50 ±0.04a 0.44 ±0.05a
24 0.71 ±0.04a 0.53 ±0.04a 0.50 ±0.04a 0.43 ±0.04a
48 0.71 ±0.05a 0.54 ±0.05a 0.50 ±0.05a 0.39 ±0.03b
Gumminess
(N)
0 5.82 ±1.36c 3.46 ±0.52c 2.68 ±0.42b 2.88 ±0.40b
6 8.65 ±1.36a 5.84 ±0.84a 4.89 ±0.66a 3.98 ±0.49a
24 8.66 ±1.27a 5.14 ±0.84b 4.73 ±0.86a 3.69 ±0.48a
48 7.54 ±1.02b 5.45 ±0.96ab 4.73 ±0.97a 3.19 ±0.34b
Chewiness
(N)
0 3.77 ±1.20b 1.62 ±0.40c 1.15 ±0.29b 1.11 ±0.27b
6 6.20 ±1.15a 3.27 ±0.67a 2.48 ±0.44a 1.77 ±0.40a
24 6.18 ±1.12a 2.75 ±0.58b 2.40 ±0.56a 1.61 ±0.33a
48 5.38 ±0.99a 2.95 ±0.80ab 2.38 ±0.71a 1.25 ±0.21b
Cohesiveness 0 0.33 ±0.08c 0.19 ±0.03b 0.15 ±0.02c 0.15 ±0.01b
6 0.39 ±0.04ab 0.24 ±0.02a 0.18 ±0.01a 0.17 ±0.01a
24 0.38 ±0.04bc 0.23 ±0.03a 0.18 ±0.01a 0.17 ±0.01a
48 0.44 ±0.10a 0.25 ±0.04a 0.16 ±0.01b 0.14 ±0.01b

Textural characteristics of cooked rice of ‘Miho’ based on storage time at -18℃.

Storage time (h) Varieties
Baekogchal Baegjinju Miho Saeilmi
Hardness
(N)
0 17.17 ±3.32b) 18.17 ±1.34c 19.69 ±2.44b 19.03 ±2.18b
6 28.34 ±5.91a 31.27 ±5.42a 29.59 ±5.98a 25.51 ±5.74a
24 24.60 ±4.41a 23.09 ±3.68b 29.25 ±5.21a 21.11 ±2.67b
48 17.41 ±5.88b 22.86 ±4.54b 29.91 ±3.80a 21.58 ±2.97b
Springiness 0 0.54 ±0.12b 0.40 ±0.05c 0.47 ±0.17a 0.36 ±0.03b
6 0.74 ±0.03a 0.62 ±0.03a 0.51 ±0.07a 0.47 ±0.07a
24 0.71 ±0.07a 0.56 ±0.06b 0.51 ±0.03a 0.43 ±0.05a
48 0.71 ±0.06a 0.53 ±0.08b 0.51 ±0.05a 0.36 ±0.10b
Gumminess
(N)
0 4.10 ±1.22d 2.83 ±0.36c 4.26 ±2.51a 2.85 ±0.35c
6 11.48 ±2.64a 8.43 ±1.33a 5.53 ±1.41a 4.47 ±1.30a
24 8.78 ±1.25b 5.55 ±1.10b 5.04 ±1.14a 3.70 ±0.68b
48 7.15 ±1.51c 5.64 ±1.81b 5.18 ±1.05a 3.13 ±0.93bc
Chewiness
(N)
0 2.34 ±1.11c 1.13 ±0.26c 2.43 ±2.18a 1.03 ±0.18c
6 8.58 ±2.18a 5.27 ±0.94a 2.90 ±1.07a 2.17 ±0.97a
24 6.27 ±1.18b 3.15 ±0.89b 2.57 ±0.71a 1.62 ±0.44b
48 5.15 ±1.35b 3.15 ±1.47b 2.67 ±0.77a 1.19 ±0.63bc
Cohesiveness 0 0.25 ±0.10c 0.16 ±0.02c 0.21 ±0.10a 0.15 ±0.01b
6 0.41 ±0.03ab 0.27 ±0.03a 0.19 ±0.02a 0.17 ±0.01a
24 0.36 ±0.03b 0.24 ±0.03b 0.17 ±0.02a 0.18 ±0.02a
48 0.44 ±0.11a 0.23 ±0.04b 0.17 ±0.02a 0.14 ±0.03b

Hunter’s color value of cooked rice ‘Miho’ based on storage time at 3℃.

Colorz Storage time (h) Varieties
Baekogchal Baegjinju Miho Saeilmi
L 0 72.84 ±1.28a) 76.27 ±0.67a 74.01 ±0.37a 77.67 ±1.21a
6 71.60 ±0.42a 76.48 ±0.65a 74.88 ±0.51a 76.46 ±0.14a
24 70.90 ±0.46a 75.70 ±0.57a 74.26 ±0.78a 75.95 ±0.43a
48 71.34 ±0.74a 76.00 ±0.35a 74.13 ±1.50a 77.45 ±1.52a
a 0 -2.00 ±0.10a -2.22 ±0.13a -2.22 ±0.07a -2.08 ±0.18a
6 -2.10 ±0.10a -2.21 ±0.14a -2.05 ±0.22a -2.04 ±0.06a
24 -2.16 ±0.10a -2.22 ±0.22a -2.16 ±0.04a -2.05 ±0.12a
48 -2.18 ±0.09a -2.30 ±0.06a -2.02 ±0.15a -2.08 ±0.22a
b 0 10.24 ±1.10b 10.31 ±0.22a 9.28 ±0.71a 8.05 ±1.24a
6 12.07 ±0.29a 10.70 ±0.99a 10.26 ±0.52a 9.40 ±0.71a
24 12.72 ±0.47a 11.18 ±0.33a 10.09 ±0.07a 10.56 ±0.66a
48 12.27 ±0.75a 11.85 ±0.76a 9.98 ±0.97a 8.99 ±1.37a

Hunter’s color value of cooked rice ‘Miho’ based on storage time at -18℃.

Colorz Storage time (h) Varieties
Baekogchal Baegjinju Miho Saeilmi
L 0 74.27 ±2.24a 74.12 ±2.69b 74.34 ±0.46b 77.13 ±0.10c
6 71.31 ±0.40b 76.23 ±0.15a 73.97 ±0.03c 77.94 ±0.51b
24 71.42 ±0.14b 76.16 ±0.31a 75.76 ±0.20a 77.79 ±0.20b
48 71.11 ±0.13b 76.87 ±0.15a 74.55 ±0.35b 78.24 ±0.58a
a 0 -2.26 ±0.06d -2.16 ±0.08c -2.11 ±0.06c -2.00 ±0.05c
6 -2.14 ±0.04c -1.98 ±0.11a -2.04 ±0.11b -1.94 ±0.05b
24 -1.77 ±0.02a -2.07 ±0.06b -1.97 ±0.04a -1.86 ±0.07a
48 -2.08 ±0.06b -2.27 ±0.07d -2.08 ±0.05bc -2.03 ±0.07c
b 0 11.41 ±0.85c 11.51 ±1.62a 7.41 ±0.28d 8.80 ±0.17c
6 12.84 ±0.71a 11.12 ±0.86a 9.07 ±0.44a 9.28 ±0.05b
24 12.68 ±0.55ab 10.83 ±0.64ab 7.70 ±0.24c 9.41 ±0.12a
48 12.38 ±0.19b 10.24 ±0.25b 8.30 ±0.44b 7.93 ±0.15d

Yield of milled rice at local adaptability test of ‘Miho’. (LAT: '14~'16)

Cultivation season Region No. of
test site
Yield of milled rice (kg/10a)
Nampyeong
(B)
Miho
(A)
Index
(A/B*100)
Yield range
(Miho)
Ordinary planting Jungbu plain 4 (526)z 556 106 487-623
Yeongnam plain 1 573 577 101 506-634
Honam plain 2 556 567 102 510-611
Average (7) 547 565ns 103
Table 1 Major agronomic traits and yield components of ‘Miho’. (LAT: '14~'16)

ns and ** mean no significant and significant at p<0.01 by T-test, respectively.

LAT: Local Adaptability Test.

Table 2 Viviparous germination and cold tolerance of ‘Miho’. (LAT: '14~'16)

ns and ** mean no significant and significant at p<0.01 by T-test, respectively.

zVVG : viviparous germination (germination rate for 7 days at 25℃).

yCold tolerance was evaluated at 17℃ irrigation nursery in Chuncheon substation, NICS.

xPA: Phenotypic Acceptability.

LAT: Local Adaptability Test.

Table 3 Response to leaf and neck blast disease of ‘Miho’. (LAT: '14~'16)

zR: resistant, M: moderate, S: susceptible.

LAT: Local Adaptability Test.

Table 4 Response to bacterial blight, virus diseases and insect pests of ‘Miho’. (LAT: '14~'16)

zRSV: Rice stripe virus, RDV: Rice dwarf virus, RBSDV: Rice black stripe dwarf virus

yBPH: Brown plant-hopper, SBPH: Small brown plant-hopper.

xR: Resistant, S: Susceptible

LAT: Local Adaptability Test.

Table 5 Grain shape of rough and brown rice of ‘Miho’. (LAT: '14~'16)

ns and * mean no significant and significant at p<0.05 by T-test, respectively.

LAT: Local Adaptability Test.

Table 6 Physiochemical properties of ‘Miho’. (LAT: '14~'16)

ns and * mean no significant and significant at p<0.05 by T-test, respectively.

LAT: Local Adaptability Test.

Table 7 Rapid viscosity analysis of ‘Miho’.

The present data were expressed mean±SD. Different letters indicate significantly different at p<0.05 by Duncan’s multiple range test.

Table 8 Water absorption ratio (%) of ‘Miho’ based on soaking time.

The present data were expressed mean±SD. Different letters indicate significantly different at p<0.05 by Duncan’s multiple range test.

Table 9 Textural characteristics of cooked rice of ‘Miho’ based on storage time at 3℃.

The present data were expressed mean±SD. Different letters indicate significantly different at p<0.05 by Duncan’s multiple range test.

Table 10 Textural characteristics of cooked rice of ‘Miho’ based on storage time at -18℃.

The present data were expressed mean±SD. Different letters indicate significantly different at p<0.05 by Duncan’s multiple range test.

Table 11 Hunter’s color value of cooked rice ‘Miho’ based on storage time at 3℃.

zL: lightness, a: redness, b: yellowness.

The present data were expressed mean±SD. Different letters indicate significantly different at p<0.05 by Duncan’s multiple range test.

Table 12 Hunter’s color value of cooked rice ‘Miho’ based on storage time at -18℃.

zL: lightness, a: redness, b: yellowness.

The present data were expressed mean±SD. Different letters indicate significantly different at p<0.05 by Duncan’s multiple range test.

Table 13 Yield of milled rice at local adaptability test of ‘Miho’. (LAT: '14~'16)

zHwaseong, ns means no significant by T-test,

LAT: Local Adaptability Test.